Lipoprotein DolP supports proper folding of BamA in the bacterial outer membrane promoting fitness upon envelope stress

In Proteobacteria, integral outer membrane proteins (OMPs) are crucial for the maintenance of envelope permeability barrier to some antibiotics and detergents. Enterobacteria, stress caused by unfolded OMPs activates sigmaE (? E ) transcriptional response. ? upregulates OMP biogenesis factors, including ?-barrel assembly machinery (BAM) that catalyses folding. Here we report DolP (formerly YraP), a -upregulated poorly understood lipoprotein, is fitness in cells undergo stress. We demonstrate interacts with BAM complex associating membrane-assembled BamA. provide evidence important proper folding BamA overaccumulates membrane, thus supporting integrity. Notably, mid-cell recruitment had been linked regulation septal peptidoglycan remodelling an unknown mechanism. now reveal that, during stress, loses its association mid-cell, thereby suggesting mechanistic link between impaired late step cell division.